Figure 4.

Erythropoiesis in the spleens of Hbb^th3/+ mice afer vamifeport treatment alone or in combination with repeated blood transfusions. (A) Gating strategy used to identify erythroid progenitors in the spleen by flow cytometry: gate I, proerythroblast; gate II, basophilic erythroblast; gate III, polychromatic erythroblast; gate IV, orthochromatic erythroblast; gate V, mature erythrocytes. Green fluorescent protein-positive (GFP⁺) transfused donor cells are excluded from the analysis. The remaining figures are representative scatter plots of individual values (with mean and standard deviation) showing the effects of transfusion, vehicle, and vamifeport treatment at the end of the study on levels of: (B) proerythroblasts, (C) basophilic erythroblasts, (D) polychromatic erythroblasts, (E) orthochromatic erythroblasts, and (F) mature erythrocytes. WT: wild-type. Analysis was performed by using one-way analysis of variance with Dunnett’s multiple comparison test. Significant differences compared with the Hbb^th3/+ vehicle non-transfused group (black) and vehicle-transfused group (purple) are indicated as: *P<0.05, **P<0.01, and ***P<0.001. Significant differences between the vamifeport-treated transfused group compared with the vamifeport-treated non-transfused group are indicated in red (unpaired t test).