Use	Species	Reagent	Clone or Catalogue Number	Company	Dilution/Concentration
Flow Cytometry	Mouse	anti-CD45	30-F11	Biolegend	1:100
Flow Cytometry	Mouse	anti-CD11c	N418	Biolegend	1:100
Flow Cytometry	Mouse	anti-I-A/I-E	M5/114.15.2	Biolegend	1:100
Flow Cytometry	Mouse	anti-TCR β	H57–597	Biolegend	1:100
Flow Cytometry	Mouse	anti-CD3	17A2	Biolegend	1:100
Flow Cytometry	Mouse	anti-CD4	RM4–5	Biolegend	1:100
Flow Cytometry	Mouse	anti-CD8a	53 – 6.7	Biolegend	1:100
Flow Cytometry	Mouse	anti-CD8b	YTS156.7.7	Biolegend	1:100
Flow Cytometry	Mouse	anti-Thy1.1	OX-7	Biolegend	1:100
Flow Cytometry	Mouse	anti-Thy1.2	30-H12	Biolegend	1:100
Flow Cytometry	Mouse	anti-CD44	IM7	Biolegend	1:100
Flow Cytometry	Mouse	anti-CD62L	MEL-14	Biolegend	1:100
Flow Cytometry	Mouse	anti-PD-1	29F.1A12	Biolegend	1:100
Flow Cytometry	Mouse	anti-CD39	Duha59	Biolegend	1:100
Flow Cytometry	Mouse	anti-Ly108 (SLAMF6)	330-AJ	Biolegend	1:100
Flow Cytometry	Mouse	anti-TIM-3	RMT3–23	Biolegend	1:100
Flow Cytometry	Mouse	anti-LAG-3	C9B7W	Biolegend	1:100
Flow Cytometry	Mouse	anti-PD-LI	10F.9G2	Biolegend	1:100
Flow Cytometry	Mouse	anti-mouse H-2Kb bound to SIINFEKL	25-D1.16	Biolegend	1:100
Flow Cytometry	Mouse	anti-TNFα	MP6-XT22	Biolegend	1:50
Flow Cytometry	Mouse	anti-IFNγ	XMG1.2	Biolegend	1:50
Flow Cytometry	Mouse	anti-GzmB	GB11	Biolegend	1:50
Flow Cytometry	Mouse	anti-IL-2	JES6–5H4	Biolegend	1:50
Flow Cytometry	Mouse	anti-Tbet	4B10	Biolegend	1:50
Flow Cytometry	Mouse	anti-Eomes	Dan11mag	eBioscience	1:50
Flow Cytometry	Mouse	anti-Ki67	11F6	Biolegend	1:50
Flow Cytometry	Mouse	anti-Tox	REA473	Militenyi Biotec	1:50
Flow Cytometry	Mouse	anti-TCF1/TCF7	C63D9	CST	1:50
Flow Cytometry	Mouse	anti-PKM2	D78A4	CST	1:50
Flow Cytometry	Mouse	anti-PKMI	D30G6	CST	1:50
Flow Cytometry	Mouse	Rabbit mAb IgG	DAIE	CST	1:50
Flow Cytometry	Mouse/Human	anti-Foxo1	W20064D	Biolegend	1:50
Flow Cytometry	Mouse/Human	anti-Bach2	ab243148	abeam	1:100
Flow Cytometry	Mouse	Goat anti-Rat AF647	A21247	Invitrogen	1:500
Flow Cytometry	Human	anti-IFNγ	4S.B3	Biolegend	1:75
Flow Cytometry	Human	anti-CD3	UCHT1	Biolegend	1:150
Flow Cytometry	Human	anti-CD8a	RPA-78	Biolegend	1:150
Flow Cytometry	Human	anti-TCF-7/TCF-1	S33–966	BD Biosciences	1:250
Viability - Flow Cytometry	N/A	Zombie Aqua	423102	Biolegend	1:100
Viability - Flow Cytometry	N/A	DAPI	422801	Biolegend	0.2μg/mL
PDTO Killing Assay	N/A	CellTrace Far Red Proliferation Kit	C34564	Thermo Fisher Scientific	1:1000
PDTO Killing Assay	N/A	NucView 488 Caspase-3 Assay Kit for Live Cells	30029	Biotium	5μM
Western Blotting	Mouse	Rabbit anti-PKMI	D30G6	CST	1:1000 in BSA
Western Blotting	Mouse	Rabbit anti-PKM2	D78A4	CST	1:1000 in BSA
Western Blotting	Mouse	Rabbit anti-PKM1/2	#3186	CST	1:500 in BSA
Western Blotting	Mouse	Rabbit anti-alpha Tubulin	#2144	CST	1:2000 in BSA
Western Blotting	Mouse	Rabbit anti-Histone H3	D1H2	CST	1:5000 in milk
Western Blotting	Rabbit	Peroxidase AffiniPure Goat Anti-Rabbit IgG (H + L)	111–035-003	Jackson Immuno-Research Laboratories	1:2000 in milk
In vivo blockade	Rat	anti-Mouse PD-1	RMP1–14	BioXCell	250μg/dose
In vivo blockade	Rat	lgG2a	2A3	BioXCell	250μg/dose
In vitro culture	Mouse	Biotinylated anti-CD3ε	145–2C11	Biolegend	2μg/mL
In vitro culture	Mouse	Biotinylated anti-CD28	37.51	Biolegend	2μg/mL
In vitro culture	Mouse	Recombinant IL-2	402-ML-100	R&D	50U/mL
In vitro culture	Chicken	OVA 257–264	vac-sin	InvivoGen	1μg/mL
In vitro culture	N/A	2-Deoxy-D-glucose	D3179–1G	Sigma-Aldrich	2mM in DMSO
In vitro culture	N/A	G6PDi-1	SML2980-5MG	Sigma-Aldrich	10–25μM in DMSO
In vitro culture	N/A	6-aminonicotinamide	A68203–1G	Sigma-Aldrich	10μM in DMSO
In vitro culture	N/A	Oxythiamine chloride hydrochloride	04000–1G	Sigma-Aldrich	10–100μM in DMSO
In vitro culture	N/A	G6PD activator AG1	HY-123962	MedChemExpress LLC	3μM in DMSO
Isotope labeling	N/A	1,2–13C2 D-Glucose	CLM-504–0	Cambridge Isotope Laboratories	2000mg/L
Human Rapid Expansion	Human	Recombinant IL-2	200–02	Peprotech	3000U/mL
Human Rapid Expansion	Human	anti-CD3	OKT3	Biolegend	1μg/mL
Enzymatic Digestion	N/A	Collagenase IV	17104019	Life Technologies	250U/mL
Enzymatic Digestion	N/A	ROCK Inhibitor	Y-27632	Selleck Chemical	10μMol/L
Human PDTO Generation	Human	Recombinant Human FGF-β	100–18B	Peprotech	1ng/mL
Human PDTO Generation	Human	Recombinant Human FGF-10	100–26	Peprotech	20ng/mL
Human PDTO Generation	N/A	PGE2	2296	R&D	1μMol/L
Human PDTO Generation	N/A	SB202190	S7067	Sigma-Aldrich	10μMol/L
Human PDTO Generation	Mouse	Recombinant Mouse EGF	μMG-8045	Gibco	50ng/mL
Human PDTO Generation	N/A	ROCK Inhibitor	Y-27632	Selleck Chemical	10μMol/L
Human PDTO Generation	Human	Recombinant Heregulin Beta-1	100–03	Peprotech	10ng/mL
Human PDTO Generation	N/A	A-83–01	2939	Tocris	500nM
Human PDTO Generation	N/A	Nicotinamide	N0636	Sigma-Aldrich	10mM
Human PDTO Generation	N/A	N-acetylcysteine	A9165–5G	Sigma-Aldrich	1.25mM
Human PDTO Generation		10% Noggin conditioned media from transfected HEK 293T	Produced in House
Human PDTO Generation		10% R-spondin conditioned media from transfected HEK 293T	Produced in House
Human PDTO Coculture	Human	Recombinant IL-2	200–02	Peprotech	300U/mL