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. 2023 Sep 30;13:16424. doi: 10.1038/s41598-023-43546-4

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Immunohistochemical staining of liver sections showing A] negative results for NF-κB in healthy control GI and vehicle group, B] marked positive results (++++) for NF-κB in injured untreated control GI, C] moderate positive result (+++) for NF-κB in injured free Mel (5 and 10 mg/kg) treated GII and GIII, D] negative results for NF-κB in injured Mel-PLGA NPs (5 and 10 mg/kg) treated GIV and GV, E] negative results for CRP in healthy control GI and vehicle group, F] marked positive results (++++) for CRP in injured untreated control GI, G] moderate positive result (+++) for CRP in injured free Mel (5 and 10 mg/kg) treated GII and GIII, H] negative results for CRP in injured Mel-PLGA NPs (5 and 10 mg/kg) treated GIV and GV.