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. 2023 Sep 15;14:1219279. doi: 10.3389/fimmu.2023.1219279

Figure 2.

Figure 2

Inhibition of Fli-1 expression decreased production of CXCL10 in human umbilical vein endothelial cells (HUVECs), human renal glomerular endothelial cells (HRGECs), and mouse endothelial MS1 cells. HUVECs (A), or HRGECS (B) were transfected with Fli-1 specific or negative control siRNA for 24 hours and further stimulated with LPS (0.2 µg/ml) or TNFα (10 ng/ml) 24 hrs. Secretion of CXCL10, CXCL9, and CXCL11 in supernatant were measured by ELISA. N=3-4 in each group. The data were represented as mean ± SEM. * indicates p < 0.05. (C) MS1 endothelial cells were transfected with Fli-1 specific or negative control siRNA for 24 hours and stimulated with LPS (0.1 µg/ml) for another 6 or 24 hours. Secreted CXCL10, CXC9 and CXCL11 concentrations were measured by ELISA. CXCL9 and CXCL11 were undetectable. N=4 in each group. The data were represented as mean ± SEM. * indicates p< 0.05.