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. 2023 Sep 15;14:1219279. doi: 10.3389/fimmu.2023.1219279

Table 1.

Primers used in ChIP assay of CXCL10 promoter.

Primer Name Forward Primer Reverse Primer Position from TSS Amplicon Length (bp)
ChiP1 5’-GAG TCA TCT CCA AAG TCA G- 3’ 5’-CAC TTG GGT TCA TGG TG-3’ -2082 to -1922 160
ChIP2 5’-GAA ACT TAC CTC ACT CG -3’ 5’-CTG ACT TTG GAG ATG ACT C-3’ -1941 to 1788 153
ChIP3 5’-GAA CCT GAC TTA GAT ATC- 3’ 5’-CCT CTT GTG CTC CTT TTA-3’ -1830 to -1660 170
ChIP4 5’-CTG CTC TAA CTG TTC AC -3’ 5’-GAT ATC TAA GTC AGG TTC-3’ -1677 to -1501 176
ChIP5 5’-CTG TAA CCA CAC ACT CAC A- 3’ 5’-GTG AAC AGT TAG AGC AG- 3’ -1518 to -1367 151
ChIP6 5’-GTT TTG AAC CGG TAC AC -3’ 5’-CTT CTT TGT GAG TGT GTG G-3’ -1391 to -1184 207
ChIP7 5’ -CTT AGC TCT GTT CTA GTC- 3’ 5’-GTG TAC CGG TTC AAA AC-3’ -1200 to -1027 173
ChIP8 5’-CTA TTC TGC AGA AGC AG-3’ 5’-GAC TAG AAC AGA GCT AAG-3’ -1027 to -858 169
ChIP9 5’-CTG AGA ACT TGT ACA ATAAC-3’ 5’-TGC TTC TGC AGA ATA GAC-3’ -873 to -733 140
ChIP10 5’-CTC TTG ACT AAC AGA TGC-3’ 5’ -GTT ATT GTA CAA GTT CTC AG-3’ -753 to -602 151
ChIP11 5’-AAG CAG ACA CAG GCA AGT-3’ 5’- CCT TAC TGA CGA GAA AAG-3’ -664 to -471 193
ChIP12 5’-CTG TTC TAG TCA AAG GG- 3’ 5’- GCT GAA GTT ATC TTT GAT G-3’ -469 to -294 175
ChIP13 5’-CTT TTA TTT CAG TCA TTT GAC-3’ 5’- GCA CTG AAT TAT AGC AGA- 3’ -285 to -129 156
ChIP14 5’-GAA TTC CGA CGT CTA CCT-3’ 5’ -GTT AAT GTC AAA TGA CTG AA-3’ -156 to -1 155

Primers are listed based on their distance from the transcription start site (TSS).