Table 1.
Primers used in ChIP assay of CXCL10 promoter.
| Primer Name | Forward Primer | Reverse Primer | Position from TSS | Amplicon Length (bp) |
|---|---|---|---|---|
| ChiP1 | 5’-GAG TCA TCT CCA AAG TCA G- 3’ | 5’-CAC TTG GGT TCA TGG TG-3’ | -2082 to -1922 | 160 |
| ChIP2 | 5’-GAA ACT TAC CTC ACT CG -3’ | 5’-CTG ACT TTG GAG ATG ACT C-3’ | -1941 to 1788 | 153 |
| ChIP3 | 5’-GAA CCT GAC TTA GAT ATC- 3’ | 5’-CCT CTT GTG CTC CTT TTA-3’ | -1830 to -1660 | 170 |
| ChIP4 | 5’-CTG CTC TAA CTG TTC AC -3’ | 5’-GAT ATC TAA GTC AGG TTC-3’ | -1677 to -1501 | 176 |
| ChIP5 | 5’-CTG TAA CCA CAC ACT CAC A- 3’ | 5’-GTG AAC AGT TAG AGC AG- 3’ | -1518 to -1367 | 151 |
| ChIP6 | 5’-GTT TTG AAC CGG TAC AC -3’ | 5’-CTT CTT TGT GAG TGT GTG G-3’ | -1391 to -1184 | 207 |
| ChIP7 | 5’ -CTT AGC TCT GTT CTA GTC- 3’ | 5’-GTG TAC CGG TTC AAA AC-3’ | -1200 to -1027 | 173 |
| ChIP8 | 5’-CTA TTC TGC AGA AGC AG-3’ | 5’-GAC TAG AAC AGA GCT AAG-3’ | -1027 to -858 | 169 |
| ChIP9 | 5’-CTG AGA ACT TGT ACA ATAAC-3’ | 5’-TGC TTC TGC AGA ATA GAC-3’ | -873 to -733 | 140 |
| ChIP10 | 5’-CTC TTG ACT AAC AGA TGC-3’ | 5’ -GTT ATT GTA CAA GTT CTC AG-3’ | -753 to -602 | 151 |
| ChIP11 | 5’-AAG CAG ACA CAG GCA AGT-3’ | 5’- CCT TAC TGA CGA GAA AAG-3’ | -664 to -471 | 193 |
| ChIP12 | 5’-CTG TTC TAG TCA AAG GG- 3’ | 5’- GCT GAA GTT ATC TTT GAT G-3’ | -469 to -294 | 175 |
| ChIP13 | 5’-CTT TTA TTT CAG TCA TTT GAC-3’ | 5’- GCA CTG AAT TAT AGC AGA- 3’ | -285 to -129 | 156 |
| ChIP14 | 5’-GAA TTC CGA CGT CTA CCT-3’ | 5’ -GTT AAT GTC AAA TGA CTG AA-3’ | -156 to -1 | 155 |
Primers are listed based on their distance from the transcription start site (TSS).