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. 2023 Oct 2;9:364. doi: 10.1038/s41420-023-01648-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A Colony formation of CAL27-CR, SCC25-CR, and SCC4-CR cells treated with various concentrations gastrodin were measured by plate colony formation assay. **p < 0.01, ***p < 0.001. B Anchorage-independent growth of CAL27-CR, SCC25-CR, and SCC4-CR cells treated with various concentrations gastrodin were detected using soft agar assay. ***p < 0.001. C The viability of OSCC cells following various inhibitors and gastrodin (40 µM) treatment was evaluated using MTS analysis. ***p < 0.001. D The number of live cells in various inhibitors- and gastrodin-treated (40 µM) OSCC cells were measured using trypan blue exclusion analysis. ***p < 0.001. The protein levels of cleaved-caspase 3 (E) and caspase 3 activity (F) in CAL27-CR and SCC4-CR cells after treatment with various doses gastrodin. ***p < 0.001. G Isolating subcellular fractions of CAL27-CR cells treated with various doses gastrodin for IB assay. HK2 was transfected into CAL27-CR cells and the cells were treated with gastrodin (40 µM) for 24 h, followed by IB analysis (H), MTS analysis (I) and Caspase 3 activity assay (J). ***p < 0.001. K CAL27-CR cells were treated with gastrodin for 24 h following transfecting HK2. Separating subcellular fractions for IB assay.