Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Oct 2;13(4):e12293. doi: 10.1002/pul2.12293

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2023 The Authors. Pulmonary Circulation published by John Wiley & Sons Ltd on behalf of Pulmonary Vascular Research Institute.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

PMC Copyright notice

Effect of macitentan, selexipag, and tadalafil on BMPRII canonical signaling pathway in HLMVECs. Representative Western blots of phosphorylated (P‐Smad) and total Smad (1/5/8) proteins in control (a) and bmpr2‐silenced HLMVECs (b) incubated with vehicle (VEH), macitentan (MACI), selexipag (SELEX) and tadalafil (TADA) for 1 h. Quantitative expression of phosphorylated versus total Smad1/5/8 proteins in control (c) and bmpr2‐silenced HLMVECs (d). Results are presented as ratio of phosphorylated over total Smad (1/5/8) protein expression. Independent experiments were performed in four replicates for HLMVECs between passages 5 and 8 and expressed as median (25th–75th interquartile range). Comparisons by one‐way ANOVA and post‐hoc Tukey's multiple tests (c, d).