Table 1.

Proportion of astrocytes (ACSA-2-PE⁺ cells), endothelia (Pecam1-APC⁺ cells), and negative (ACSA-2-PE⁻/ Pecam1-APC⁻) cells as determined by FACS. Cultured cells were dissociated and labeled with anti-ACSA-2 antibodies coupled to the fluorophore PE and with anti-Pecam1 antibodies coupled to APC and subjected to FACS (see methods section for details). Cells were collected from a gate that excluded debris and aggregates. 20,000 events were collected per experiment, and the percentage of cells was calculated as follows: astrocytes all PE⁺ cells, endothelia all APC⁺ cells, and negative cells (negative cells = total cells - PE⁺ - APC⁺). The mean and standard deviation of five experiments (six samples per experiment) are shown.

	A (Mean)	A (STDEV)	E (Mean)	E (STDEV)	Neg (Mean)	Neg (STDEV)
A	94.47	4.4	0.01	0.0	5.51	4.4
EA	42.03	12.2	54.40	11.3	3.56	1.7
AN	71.94	11.1	0.00	0.0	28.06	11.1
EAN	20.79	8.6	41.89	5.7	37.32	6.2
E	0.01	0.0	97.45	1.4	2.54	1.4
N	0.57	0.2	0.13	0.1	99.30	0.3