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. 2023 Aug 22;8(16):e165944. doi: 10.1172/jci.insight.165944

Figure 1. Deficiency of GlcNAc-6-O-sulfation promotes age-associated obesity.

Figure 1

(A) Weight gain of WT and Chst4–/– mice measured over a 15-month period. (B) Representative image of 15-month-old WT and Chst4–/– mice. (C) Total weight of 15-month-old WT and Chst4–/– mice (WT n = 14, Chst4–/– n = 12). (D) Liver weight of WT and Chst4–/– mice (WT n = 11, Chst4–/– n = 8). (E) Representative image of epididymal white adipose tissue (eWAT) from WT and Chst4–/– mice. (F) Adipose tissue weight (n = 8). (G) Representative H&E and Oil Red O staining of liver and adipose tissue from WT and Chst4–/– mice. Scale bar: 200 μm. (H) Fifteen-hour fasting blood glucose concentrations in WT and Chst4–/– mice at 15 months. (I) Blood glucose concentration in glucose tolerance tests (n = 8). (J) Change in blood glucose concentration in WT and Chst4–/– mice after additional insulin challenge (WT n = 10, Chst4–/– n = 9). Data are representative of 2 independent experiments, and presented as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 via 2-way ANOVA followed by Tukey’s multiple-comparison test (A, I, and J) or unpaired, 2-tailed t test (C, D, F, and H).