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. 2023 Aug 22;8(16):e165944. doi: 10.1172/jci.insight.165944

Figure 6. Altered microbiota accelerates weight gain in Chst4–/– mice.

Figure 6

(A) Weight gain of WT and Chst4–/– mice treated with antibiotics (Abx) measured for 6 weeks. (B) Total weight of WT and Chst4–/– mice fed an HFD with antibiotics. (C) Adipose tissue weight in WT and Chst4–/– mice (WT n = 7, WT + Abx n = 6, Chst4–/– n = 7, Chst4–/– + Abx n = 7). (D) Representative H&E and Oil Red O staining of liver and adipose tissue from WT and Chst4–/– mice treated with antibiotics. (E) PCoA analysis of ileal bacteria using unweighted UniFrac distance, after 3 weeks of cohousing. (F) PCoA analysis of fecal bacteria using unweighted UniFrac distance, after 3 weeks of cohousing (n = 4). (G) Representative images of 6-week-old WT and Chst4–/– mice fed an HFD in the cohousing experiment. (H) Weight gain measured over 6 weeks of HFD feeding with cohousing. (I) Total weight of WT and Chst4–/– mice. (J) Adipose tissue weight (WT n = 10, WT/cohoused n = 8, Chst4–/– n = 8, Chst4–/–/cohoused n = 8). (K) Representative images of Oil Red O– and H&E-stained liver tissues from WT and Chst4–/– mice after 6 weeks of HFD feeding with cohousing. Scale bars: 200 μm. Data are representative of 2 independent experiments, and presented as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 via 2-way ANOVA followed by Tukey’s multiple-comparison test (A and H) or 1-way ANOVA (B, C, I, and J). eWAT, epididymal white adipose tissue.