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. 2023 Sep 29;21:353. doi: 10.1186/s12951-023-02104-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — a CaCO₃@Cur@QTX125@HA Image of the Z-stack in the PDO model of colorectal cancer. Blue: DAPI-labeled nucleus, red: nanoparticles labeled with RhB). Scale bar: 50 μ m; b Representative confocal images of PDO models after treatment with CaCO₃, CaCO₃@Cur@QTX125 and CaCO₃@Cur@QTX125@HA nanoparticles, measured by TUNEL assay. c Expression of tumor marker CEA in CRC PDO model after incubation with CaCO₃, CaCO₃@Cur@QTX125 and CaCO₃@Cur@QTX125 @HA nanoparticles at a Cur concentration of 20 μg/mL; d Expression of tumor marker CA19-9 in CRC PDO model after incubation with CaCO₃, CaCO₃@Cur@QTX125 and CaCO₃@Cur@QTX125@HA nanoparticles at a Cur concentration of 20 μg/mL. The nuclei were labeled with DAPI in blue, Cur were labeled with Rhodamine B in red, and tumor markers were labeled with AlexaFluor^® 488 in green. The scale bars represent 50 μm