Table 2.

Repeatability of the assay in one run (5 repetitions) and across 3 runs (15 repetitions)

PCR	Mean Ct	SD	СV (%)
Run 1	29.68	0.97	3.27
Run 2	30.26	1.1	3.64
Run 3	29.93	0.56	1.87
n = 15
Across runs	29.96	0.88	2.94

PCR: polymerase chain reaction; Ct: cycle threshold; SD: standard deviation; CV: coefficient of variation