Table 1.
Echocardiographic Parameters in 36-Week-Old Mice
| Transgene |
Genotype |
||||||
|---|---|---|---|---|---|---|---|
| Bmal1 Flox |
+ |
+ |
− |
− |
+ |
+ |
|
| E4bp4 Flox |
− |
− |
+ |
+ |
+ |
+ |
|
| MHCα-Cre | − | + | − | + | − | + | |
| IVSd, mm | 0.86 ± 0.03 | 0.82 ± 0.03 | 0.70 ± 0.02 | 0.72 ± 0.02 | 0.82 ± 0.04 | 0.88 ± 0.04 | |
| IVSs, mm | 1.22 ± 0.04 | 1.00 ± 0.04∗∗∗ | 0.96 ± 0.03 | 0.96 ± 0.02 | 1.12 ± 0.05 | 1.20 ± 0.05†† | |
| LVIDd, mm | 4.04 ± 0.08 | 4.62 ± 0.13∗∗ | 3.95 ± 0.08 | 4.22 ± 0.09 | 4.03 ± 0.11 | 4.13 ± 0.09† | |
| LVIDs, mm | 2.95 ± 0.11 | 4.06 ± 0.16∗∗∗ | 3.02 ± 0.08 | 3.30 ± 0.11 | 3.05 ± 0.07 | 3.22 ± 0.12†† | |
| LVPWd, mm | 0.78 ± 0.02 | 0.85 ± 0.03 | 0.74 ± 0.02 | 0.74 ± 0.02 | 0.90 ± 0.06 | 0.90 ± 0.03 | |
| LVPWs, mm | 1.04 ± 0.03 | 0.95 ± 0.04 | 0.98 ± 0.03 | 0.96 ± 0.02 | 1.13 ± 0.08 | 1.09 ± 0.04 | |
| EF, % | 52.8 ± 2.78 | 26.8 ± 2.62∗∗∗ | 47.6 ± 1.57 | 44.1 ± 2.32 | 48.4 ± 2.65 | 44.7 ± 2.71†† | |
| FS, % | 27.1 ± 1.70 | 12.5 ± 1.33∗∗∗ | 23.6 ± 0.89 | 21.8 ± 1.35 | 24.2 ± 1.61 | 22.1 ± 1.51†† | |
| LV Vold, μL | 72.3 ± 3.47 | 99.8 ± 6.34∗∗ | 68.4 ± 3.52 | 80.4 ± 4.15 | 71.6 ± 4.88 | 76.2 ± 4.28† | |
| LV Vols, μL | 34.6 ± 3.19 | 74.7 ± 6.86∗∗∗ | 36.0 ± 2.48 | 45.4 ± 3.70 | 36.6 ± 2.21 | 42.9 ± 4.50†† | |
Echocardiographic parameters in 36-week-old cardiomyocyte-specific BMAL1 knockout (CBK), cardiomyocyte-specific E4BP4 knockout (CEK), CBK/CEK double knockout, and littermate flox control mice are shown. All data are presented as mean ± SEM.
EF = ejection fraction; FS = fractional shortening; IVSd = inner ventricular septal wall thickness during diastole; IVSs = inner ventricular septal wall thickness during systole; LVIDd = left ventricular inner diameter during diastole; LVIDs = left ventricular inner diameter during systole; LVPWd = left ventricular posterior wall thickness during diastole; LVPWs = left ventricular posterior wall thickness during systole; LV Vold = left ventricular volume during diastole; LV Vols = left ventricular volume during systole.
P < 0.01 and ∗∗∗P < 0.001 for MHCα-Cre–positive vs MHCα-Cre–negative littermate floxed control.
P < 0.05 and ††P < 0.01 for CBK vs CBK/CEK double knockout.