Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Feb 10;23(6):2487–2502. doi: 10.1007/s10238-023-01012-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — Rhabdomyosarcoma cell lines expressed iron-related proteins and genes. A Western blot analysis for Transferrin Receptor1 (TfR1), Ferroportin (FPN) in cell lines representing human Embryonal (ERMS: RD, SMS, RH36) and Alveolar (ARMS: RH4, RH18, RH30) rhabdomyosarcoma. Tubulin was used as loading control (N = 3). B ELISA assay for H- and L- ferritin in cell lines representing human ERMS and ARMS (N = 3). C–E qPCR for Zip14 (C), NCOA4 (D) and Hepcidin (E) mRNAs in cell lines representing human ERMS and ARMS. The mRNA levels are expressed as 2^(−dCt) related to GAPDH (N = 3). HepG2 cell line was used as internal control for the analysis in B, C, D, E, since it is derived from liver, one of the main tissues involved in iron metabolism