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. 2023 Aug 1;83(19):3192–3204. doi: 10.1158/0008-5472.CAN-23-0285

Figure 2.

Figure 2. Genome-wide analyses link AR to metabolism-related biological processes. A, Heatmap showing expression of genes downregulated (left) and upregulated (right) by DHT treatment relative to mock in LNCaP cells cultured in medium containing charcoal-stripped serum (CSS). B, GO enrichment analysis of the 415 upregulated genes [log2 (fold change) > 1] using the tools from the GSEA website (https://www.gsea-msigdb.org/gsea/msigdb/human/annotate.jsp). Top 10 annotation clusters are shown according to their enrichment scores [−log10 (P value)]. C, Diagram showing the role of SLC7A11 in the context of ferroptosis regulation. D, UCSC Genome Browser screenshot of RNA-seq data showing SLC7A11 expression levels in LNCaP (GSM2432771 vs. GSM2432769) and C4–2 cells (GSM2432783 vs. GSM2432781) cultured in CSS medium and treated with or without DHT (10 nmol/L) for 24 hours. E and F, qRT-PCR (E) and Western blot (F) analysis of SLC7A11 expression in LNCaP and C4–2 cells treated as in D. G–I, UCSC screenshot of RNA-seq data (G) showing SLC7A11 expression levels in LNCaP cells (GSM6132392 vs. GSM6132398) treated with or without ENZ (10 μmol/L) for 72 hours and qRT-PCR (H) and WB (I) analysis of SLC7A11 expression in LNCaP and C4–2 cells with the same treatments as in G. J and K, Western blot (J) and qRT-PCR (K) analysis of SLC7A11 protein (J) and mRNA expression (K) in LNCaP and C4–2 cells infected with lentivirus expressing nonspecific shRNA (shNS) or AR-specific shRNAs for 72 hours. L and M, qRT-PCR (L) and Western blot (M) analysis of LuCaP35 xenograft tumors from male mice (n = 5 mice/group) treated with sham castration or castration for 1 week. N, The dot plots showing the comparison of expression levels of AR (left) and SLC7A11 (right) between primary tissues and corresponding metastatic prostate cancer patient samples (GSE32269). O, The scatter plots showing the positive correlation between AR and SLC7A11 mRNA expression in GSE32269 dataset. P and Q, Representative images (P) and quantitative data (Q) for SLC7A11 protein IHC staining in a group of primary tissues (n = 20) and metastatic CRPC samples (n = 20).

Genome-wide analyses link AR to metabolism-related biological processes. A, Heatmap showing expression of genes downregulated (left) and upregulated (right) by DHT treatment relative to mock in LNCaP cells cultured in medium containing charcoal-stripped serum (CSS). B, GO enrichment analysis of the 415 upregulated genes [log2 (fold change) > 1] using the tools from the GSEA website (https://www.gsea-msigdb.org/gsea/msigdb/human/annotate.jsp). Top 10 annotation clusters are shown according to their enrichment scores [−log10 (P value)]. C, Diagram showing the role of SLC7A11 in the context of ferroptosis regulation. D, UCSC Genome Browser screenshot of RNA-seq data showing SLC7A11 expression levels in LNCaP (GSM2432771 vs. GSM2432769) and C4–2 cells (GSM2432783 vs. GSM2432781) cultured in CSS medium and treated with or without DHT (10 nmol/L) for 24 hours. E and F, qRT-PCR (E) and Western blot (F) analysis of SLC7A11 expression in LNCaP and C4–2 cells treated as in D. G–I, UCSC screenshot of RNA-seq data (G) showing SLC7A11 expression levels in LNCaP cells (GSM6132392 vs. GSM6132398) treated with or without ENZ (10 μmol/L) for 72 hours and qRT-PCR (H) and WB (I) analysis of SLC7A11 expression in LNCaP and C4–2 cells with the same treatments as in G. J and K, Western blot (J) and qRT-PCR (K) analysis of SLC7A11 protein (J) and mRNA expression (K) in LNCaP and C4–2 cells infected with lentivirus expressing nonspecific shRNA (shNS) or AR-specific shRNAs for 72 hours. L and M, qRT-PCR (L) and Western blot (M) analysis of LuCaP35 xenograft tumors from male mice (n = 5 mice/group) treated with sham castration or castration for 1 week. N, The dot plots showing the comparison of expression levels of AR (left) and SLC7A11 (right) between primary tissues and corresponding metastatic prostate cancer patient samples (GSE32269). O, The scatter plots showing the positive correlation between AR and SLC7A11 mRNA expression in GSE32269 dataset. P and Q, Representative images (P) and quantitative data (Q) for SLC7A11 protein IHC staining in a group of primary tissues (n = 20) and metastatic CRPC samples (n = 20).