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. 2023 Aug 1;83(19):3192–3204. doi: 10.1158/0008-5472.CAN-23-0285

Figure 4.

Figure 4. Role of SLC7A11 in AR-mediated prostate cancer cell growth. A–D, LNCaP and C4–2 cells transfected with shNS or shSLC7A11 and stable cell lines were used for Western blot analysis (A), MTS assays (B), and colony formation assays, followed by photographing (C) and quantification (D). Three biological replicates were analyzed. ERK2 was used as a loading control. E–H, LNCaP cells were infected with lentivirus expressing the indicated shRNAs or expression vectors for 72 hours and subjected to Western blot analysis (E), MTS assays (F), and colony formation assays, followed by photographing (G) and quantification (H). Three biological replicates were analyzed. I–L, LNCaP cells infected with lentivirus expressing the indicated plasmids were treated with or without ENZ and subjected to Western blot analysis (I), MTS assays (J), and colony formation assays, followed by photographing (K) and quantification (L). Three biological replicates were analyzed.

Role of SLC7A11 in AR-mediated prostate cancer cell growth. A–D, LNCaP and C4–2 cells transfected with shNS or shSLC7A11 and stable cell lines were used for Western blot analysis (A), MTS assays (B), and colony formation assays, followed by photographing (C) and quantification (D). Three biological replicates were analyzed. ERK2 was used as a loading control. E–H, LNCaP cells were infected with lentivirus expressing the indicated shRNAs or expression vectors for 72 hours and subjected to Western blot analysis (E), MTS assays (F), and colony formation assays, followed by photographing (G) and quantification (H). Three biological replicates were analyzed. I–L, LNCaP cells infected with lentivirus expressing the indicated plasmids were treated with or without ENZ and subjected to Western blot analysis (I), MTS assays (J), and colony formation assays, followed by photographing (K) and quantification (L). Three biological replicates were analyzed.