Tablee 3.
Knockout strategies for overproduction of Ethanol, Lactate and 2,3 Butanediol on syngas predicted by OptKnock with 1, 2, and 3 deletions, compared to wild type. In parenthesis is reported the % variation with respect to the wild type.
| Desired product | Target reactions | Growth rate (h-1) | Product flux (mmol/gCDW/h) | CO2 emission flux (mmol/gCDW/h) |
|---|---|---|---|---|
| Ethanol | Wild type | 0.068 | 4.83 | 17.48 |
| KAS15 | 0.052 (−23.5%) | 5.07 (+5.0%) | 17.62 (+0.8%) | |
| KAS15, RNDR1 | 0.050 (−26.5%) | 5.13 (+6.2%) | 17.65 (+1.0%) | |
| ACALD, PTAr, LDH | 0.047 (−30.9%) | 5.67 (+17.4%) | 19.61 (+12.2%) | |
| Lactate | Wild type | 0.068 | 0 | 17.48 |
| ACALD, ACKr | 0.048 (- 29.4%) | 5.77 | 14.31 (−18.1%) | |
| ACALD, ACKr, GLUDy | 0.045 (−33.8%) | 5.91 | 14.59 (−16.5%) | |
| 2,3 Butanediol | Wild type | 0.068 | 0 | 17.48 |
| LDH, ACKr, ALCD2x | 0.036 (−47.1%) | 3.50 | 20.82 (+19.1%) |