Table 1.
Summary of differences between TCT and TCT-LS processes
| Process parameter | TCT process | TCT-LS process |
|---|---|---|
| Maximum chamber capacity (mL) | 250 | 600 |
| Maximum chamber capacity (cell number) | 5 × 109 | ≥ 1.5 × 1010a |
| Maximum number of target cells for selection | 3 × 109 | 3 × 109 |
| Selection reagent | CD4/CD8 microbeads (1 vial each) | CD4/CD8 microbeads (1 vial each) |
| Number of CD4+/CD8+ cells to start culture | 1 × 108 | 4 × 108 |
| Activation reagent | MACS GMP T cell TransAct (1 vial) | MACS GMP T cell TransAct – Large Scale (1 vial) |
| Cell culture conditions | Static culture until day 3 | Shaking from day 0 |
a
Greater cell numbers may be possible with further optimization of culture conditions.