Figure 4.

Autophagy Protein Expression Increases Post–Left Ventricular Assist Device

(A) Representative Western blots for Beclin-1 and VPS34. (B and C). Normalized expression quantification for Beclin-1 (B) and VPS34 (C). (D) Representative Western blots for ATG5-12 conjugate, Gabarapl1, and LC3-II. (E to G) Normalized expression quantification for ATG5-12 (E), Gabarapl1 (F), and LC3-II (G). (H) Representative Western blots for P62, BNIP3, Parkin, and PINK1. (I to L). Normalized expression quantification for P62 (I), BNIP3 (J), Parkin (K), and PINK1 (L). (M). Representative western blots for cathepsin D and Lamp1. (N, O). Normalized expression quantification for cathepsin D (N) and Lamp1 (O). For all, expression was normalized to either GAPDH or Total Protein, n = 16 pre–left ventricular assist device, 16 post–left ventricular assist device; data were analyzed by two-tailed paired Student's t-test; ∗P < 0.05, ∗∗P < 0.01, ∗∗∗∗P < 0.0001, ns = not significant