Figure 5.

Ubiquitinated Protein Levels Decrease Post–Left Ventricular Assist Device

(A and B) Representative Western blot for pan-ubiquitin pre- and post-LVAD (A) and quantification normalized to total protein signal (B). (C and D) Representative Western blot for K48-linked poly-ubiquitination pre- and post-LVAD (C) and quantification normalized to total protein signal (D). (E and F) Representative Western blot for K63-linked poly-ubiquitination pre- and post-LVAD (E) and quantification normalized to total protein signal (F). For all, n = 16 pre–left ventricular assist device, 16 post–left ventricular assist device; data analysis by 2-tailed paired Student's t-test; ∗P < 0.05, ∗∗P < 0.01.