Fig. 3.

E6446 inhibits adipogenic differentiation and hepatic lipogenesis by targeting SCD1. OP9 and AML12 cells were transfected with the pcDNA3.1 vector or pcDNA3.1-SCD1. After transfection for 24 h, OP9 cells were incubated with 1 μM rosiglitazone for 15 days to induce adipogenic differentiation. Effect of E6446 (10 μM) on A SCD1 protein expression, B Oil Red O staining (scale bar: 200 μm, 250 μm), C cellular TG level, and D mRNA expression of adipogenic differentiation -related genes. After transfection for 24 h, AML12 cells were treated with a combination of palmitic and oleic acid (PAOA) for 48 h. Effect of E6446 (10 μM) on E SCD1 protein expression, F Oil Red O staining (scale bar: 200 μm, 250 μm), G cellular TG level, and H mRNA expression of lipogenesis-related genes. The values presented are the means ± SEMs of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 vs. the Control group. ^#P < 0.05, ^##P < 0.01, ^###P < 0.001, vs. the E6446 + pcDNA3.1 group