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. 2023 Sep 29;11:e16107. doi: 10.7717/peerj.16107

Table 1. Summary of the OJIP-test parameters using data extracted from the fast fluorescence transient.

Fluorescence parameter Calculation
Fo Minimal fluorescence when all photosystem II reaction centers are open (at 50millisecond)
Fj Fluorescence intensity at the J-step (at 2 millisecond)
Fi Fluorescence intensity at the I-step (at 30 millisecond)
Fm (Fp) Maximal fluoresxence intensity when all photosystem II reaction centers are closed
Vj Relative variable Chl fluorescence (at 2 millisecond) (FJ −Fo)/(FM −Fo)
Vi Relative variable Chl fluorescence (at 30 millisecond) (FI −Fo)/(FM −Fo)
Fv/Fm Maximum quantum yield of PSII photochemistry measured in the dark-adapted state (Fm −F0)/Fm
PIABS Performance index on an absorption basis: the efficiency of energy conservation from absorbed photons to a reduction in intersystem electron carriers (RC/ABS) [(ΦPo/(1 −ΦPo)] [(1 −VJ)/(1 −(1 −VJ))]
Mo Slope at the beginning of the transient FO → FM, maximal fractional rate of photochemistry approximated initial slope (per millisecond) of the fluorescence transient V = f(t). This parameter reflects the rate at which photosystem II reaction centers are closed. It is a net value, because reduced QA can be reoxidized as a result of electron transport outside QA. 4 (F300 −Fo )/(FM −Fo)
Ss Normalized total area above the OJIP curve (reflecting multiple turnover of QA reductions). The Ss minimum appears only when each QA is reduced only once the smallest SM turn-over (single turn-over) Area/(FM −Fo)
Specific fluxes per active PSII reaction center
ABS/RC Average absorbed photon flux per photosystem II reaction center Mo (1/VJ) (1/ ΦPo)
TRo/RC Energy flux trapped by one active photosystem II reaction center Mo (1/VJ)
ETo/RC Rate of electron transport flux from QA to QB per photosystem II reaction center Mo (1/VJ) (1 −VJ)
DIo/RC Energy flux not intercepted by a reaction center ABS/RC −TRo/RC
REo/RC Electron flux leading to a reduction in the PSI end acceptor Mo (1/VJ) (1 −VI)
ΦPo Quantum yield of primary photochemistry; probability that an absorbed photon leads to a reduction in QA (FM −Fo)/FM