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. 2023 Sep 18;11:1261048. doi: 10.3389/fcell.2023.1261048

FIGURE 4.

FIGURE 4

Quantification of γA3 stub, γA3 stub Δ164 and γA3 stub Δ171 ubiquitination. (A) Pcdh-γA3 VCD sequence with indicated deletions analyzed in the stub backbone. The segment required for ubiquitination is underlined. (B) γA3 stub, γA3 stub Δ164 and γA3 stub Δ171 immunoprecipitates were probed for anti-ubiquitin and GFP. Quantification of ubiquitin smear relative to GFP is shown at right. **p <0.005 (C) Cotransfection of γA3 stub, γA3 stub Δ164 and γA3 stub Δ171 with ubiquitin-RFP. Quantification of colocalization is shown at right. ***p <0.001.