Table 1.
Summary of potential probe hybridization derived from in-silico validation. Probes were aligned with 383 fungal reference genomes available when probes were created, and an additional 100 new reference genomes available at the end of 2022
| Database access date | Phylum | Number of assemblies per phylumb | Number of hybridized assembliesc | Number of aligned probesd | Average number of hits per phylum | |
|---|---|---|---|---|---|---|
| July, 2021a | Ascomycota | 295 | 295 | 618 | 629 | |
| Basidiomycota | 70 | 70 | 241 | 252 | ||
| Chytridiomycota | 3 | 3 | 123 | 129 | ||
| Microsporidia | 10 | 10 | 64 | 65 | ||
| Mucoromycota | 4 | 4 | 208 | 333 | ||
| Zoopagomycota | 1 | 1 | 260 | 334 | ||
| Dec, 2022e | Ascomycota | 76 | 76 | 652 | 656 | |
| Basidiomycota | 13 | 13 | 127 | 147 | ||
| Microsporidia | 3 | 1 | 2 | 2 | ||
| Mucoromycota | 7 | 7 | 130 | 239 | ||
| Zoopagomycota | 1 | 1 | 80 | 92 | ||
a Three genomes (GCF_000149205.2, GCF_000149645.2, and GCF_000143185.1) are suppressed from this analysis due to the detection of contamination.
b Number of assemblies used to assess the number of hits within fungal genomes.
c Number of assemblies with at least 1 hit having more than 85% sequence match between the probe and genome sequence over a 102-bp (85%) alignment.
d Average number of unique probes that aligned with the assemblies.
e Additional 100 genomes incorporated into fungal RefSeq database.