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. 2023 Oct 2;21:271. doi: 10.1186/s12964-023-01273-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — LINC00707 resides mostly in the cytoplasm. A Schematic representation of the genomic location of the LINC00707 gene, together with its neighboring genes at chromosome 10. Arrows point to gene transcripts along with the direction of their transcription. B RNA-FISH for LINC00707 combined with immunofluorescence for lamin-B1 in HaCaT cells. The arrows depict specific LINC00707 RNA spots. Magnification bars (10 μm). C, D RNAscope for transiently overexpressed LINC00707 in PC3U cells (C) and for endogenous LINC00707 in U3034MG cells (D). The arrows depict specific LINC00707 RNA spots. Magnification bars (10 μm). E RT-qPCR for determination of LINC00707 in nuclear and cytoplasmic fractions of HaCaT, PC3U and U3034MG lysates. The graphs present data from (n = 2) biological repeats, each with three technical repeats, as percentage of total (nuclear plus cytoplasmic) RNA expression with SEM