Fig. 5.

LINC00707 negatively regulates tumor cell invasion. A, B Transwell-based collagen invasion assays of PC3U cells transiently transfected with siRNA targeting LINC00707 or control siRNA (A) or transiently transfected with pcLINC00707 or control pcDNA3 (B). Cells were treated or not with TGFβ for 18 h during the invasion period. C-F Transwell-based laminin invasion assays of U3031MG (C, D) or U3034MG (E, F) cells transiently transfected with siRNA targeting LINC00707 or control siRNA (C, E) or stably transfected with pcLINC00707 (pcLINC1, pcLINC2) or control pcDNA3 (D, F). Cells were treated or not with TGFβ for 18 h. The graphs present data from (n = 6, PC3U; n = 2, U3031MG; n = 3, U3034MG) biological replicates, each with three technical replicates, and each replicate representing the sum of 10 random areas of each transwell filter. Statistical significance of the variables was assessed by ANOVA test with Bonferroni correction and is presented as means with SEM and associated significance as: * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Representative photomicrographs are also shown indicating DAPI-positive nuclei. Magnification bars (50 μm). The difference in nuclear size is due to the comparison of different cell types (prostate cancer PC3U cells or brain tumor cells (U3031MG, U3034MG)). The difference in nuclear size between panels of the same cell line is due to the different cell density on each cell culture; dense cultures present nuclei with “smaller” and sparse cultures present nuclei with “larger” circumference as observed under 2D imaging