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. 2023 Oct 2;21:271. doi: 10.1186/s12964-023-01273-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — LINC00707 negatively regulates TGFβ-regulated gene expression. A, B RT-qPCR analysis for PAI1, N-cadherin and vimentin in PC3U cells transiently transfected with siRNA targeting LINC00707 or control siRNA (A), or transiently transfected with pcLINC00707 or control pcDNA3 (B). C, D Immunoblot and quantification for the indicated proteins (fibronectin, ZEB1, PAI1, N-cadherin) in PC3U cells transiently transfected with siRNA targeting LINC00707 or control siRNA (C), or in PC3U cells transiently transfected with pcLINC00707 or control pcDNA3 (D). Cells were treated or not with TGFβ for 24 h (A-D). GAPDH serves as loading control and molecular size markers in kDa are shown. The graphs present data from (n = 3, each with three technical repeats for RT-qPCR and n = 6 for immunoblots) biological replicates as means with SEM and associated significance as: * p < 0.05, ** p < 0.01, *** p < 0.01, **** p < 0.0001, ns: not significant difference. Statistical significance of the variables was assessed by ANOVA test with Bonferroni correction