TABLE 3.
Multiple-sequence alignment for the Ω-loop region in the class A β-lactamases
| Source | Sequence of Ω loop, locus IVa |
|---|---|
| * * | |
| B. fragilis CS30 | 162-VIHTENDMHKNLEFCYQNWTTP-183 |
| B. uniformis WAL-7088 | 162-LSETEDGMHSSFEAVYRNWSTP-183 |
| PER-1, P. aeruginosa RNL-1 | 167-VVANEAQMHADDQVQYQNWTSM-188 |
| B. vulgatus CLA341 | 180-IAYTEEEMSADHNKAYSNYTSP-201 |
| OHIO-1, E. cloacae | 158-LDRWETELNEALPGDARDTTTA-179 |
| SHV-1, K. pneumoniae | 158-LDRWETELNEALPGDARDTTTP-179 |
| LEN-1, K. pneumoniae LEN-1 | 158-LDRWETALNEALPGDARDTTTP-179 |
| TEM-1, E. coli | 160-LDRWEPELNEAIPNDERDTTMP-181 |
| P. mirabilis GN179 | 165-LDRWEPELNEAVPGDKRDTTTP-186 |
| PSE-4, P. aeruginosa Dalgleish | 157-LDRIEPDLNEGKLGDLRDTTTP-178 |
| R. capsulatus SP108 | 166-LDRIEPKLNDFASGDERDTTSP-187 |
| NMC, E. cloacae NOR-1 | 164-LDRWELDLNTAIPGDERDTSTP-185 |
| Sme-1, S. marcescens S6 | 166-LDRWELELNTAIPGDKRDTSTP-187 |
| OXY-2, K. oxytoca D488 | 164-LDRTEPTLNTAIPGDERDTSTP-185 |
| K. oxytoca E23004/SL781/SL7811 | 165-LDRTEPALNSAIPGDKRDTTTP-186 |
| S. typhimurium CAS-5 | 165-LDRTEPTLNTAIPGDPRDTTTP-186 |
| MEN-1, E. coli MEN | 137-LDRTEPTLNTAIPGDPRDTTSP-158 |
| S. fonticola CUV | 141-LDRTEPTLNTAIPGDKRDTTSP-162 |
| C. diversus ULA27 | 168-LDRKEPELNTAIPGDERDTTCP-189 |
| P. vulgaris 5E78-1 | 167-LDRKEPELNTAIHGDPRDTTSP-188 |
| B. cepacia 249 | 175-LDRWETELNTALPGDLRDTTTP-196 |
| Y. enterocolitica serotype O:3/Y-56 | 167-LDRWEPDLNTARPNDPRDTTTP-188 |
| M. tuberculosis H37RV | 178-LDAEEPELNRDPPGDERDTTTP-199 |
| S. clavuligerus NRRL 3585 | 186-MDQYEPELNHDPPHDPRDTTTP-207 |
| III, B. cereus 569/H | 185-SERFEPELNEVNPGETHDTSTP-206 |
| B. licheniformis 749/C | 176-PERFEPELNEVNPGETQDTSTA-197 |
| I, B. mycoides NI10R | 179-SDRFETELNEAIPGDIRDTSTA-200 |
| I, B. cereus 569/H/9 | 181-SNRFETELNEAIPGDIRDTSTA-202 |
| I, B. cereus 5/B | 179-SDRFETELNEAIPGDIRDTSTA-200 |
| B. subtilis 168/6GM | 179-AKRFETDLNSAIPGDIRDTSTA-200 |
| 2, S. cacaoi DSM40057 | 186-MERVEPFLSRWEPGSKRDTSTP-207 |
| S. badius DSM40139 | 184-MDREEPELSRWVPGEKRDTSTP-205 |
| Actinomadura sp. strain R39 | 176-ADRIETELNEVPPGETRDTSTP-197 |
| N. lactamdurans LC411 | 175-ADRWEPELNSAVPGDVRDTSTP-196 |
| S. cacaoi KCC S0352 | 194-MDRYEQELGSAVPGDPRDTSTP-215 |
| ROB-1, H. influenzae F990/LNPB51/A1 | 176-TNRLEPDLNQAKPNDIRDTSTP-197 |
| S. fradiae DSM40063 | 178-LDRWEPELNSAEPGRVTDTTSP-199 |
| S. lavendulae DSM2014 | 178-LDRWEPELNSAEPGRLTDTTTP-199 |
| S. albus G | 187-LDRWEPELNSAEPGRVTDTTSP-208 |
| S. lavendulae KCCS0263 | 176-LDRWEPELNSAEPDRVTDVTTP-197 |
| S. aureofaciens | 183-LDRWEPALNSAEPDRVTDTTSP-204 |
| S. cellulosae KCCS0127 | 180-LDRWEPELNSGEPDRRTDTTSP-201 |
| M. fortuitum | 167-LDRWEVELNSAIPGDPRDTSTA-188 |
| S. aureus PC1/SK456/ NCTC 9789 | 153-PVRYEIELNYYSPKSKKDTSTP-174 |
| BRO-1, M. catarrhalis ATCC 53879 | 184-LANNEPLLNRSHYGETSDTAKP-205 |
a
The numbering system for the sequences is according to the sequence for each protein as deposited in the GenBank or SWISS-PROT data banks. Positions corresponding to Glu-166 and Asn-170, respectively, are marked with asterisks.