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. Author manuscript; available in PMC: 2024 Sep 1.
Published in final edited form as: J Struct Biol. 2023 Jul 4;215(3):107995. doi: 10.1016/j.jsb.2023.107995

Figure 2.

Figure 2.

Cryo-EM image processing of F-actin decorated with smooth muscle HMM. (A) An electron micrograph showing F-actin decorated with HMM. (B) Showing representative good class averages resulted from 2D classification in Relion performed with and without imposed helical symmetry. Red arrows show an estimate of the diameter of the filaments to be approximately 380Å. (C) Result of local resolution determination using Local MonoRes (Vilas et al., 2018) shown as a heatmap of the refinement after selecting the best classes from unmasked 3D classifications. (D) The 3D soft-edge mask (shown in grey) used for 3D classifications. This mask includes both motor domains and the blurred lever arms (shown in cyan). (E) The best Acto-HMM density map, 3D reconstructed by only including the segments from group 1. (F) Magnified view of the segmented map in E.