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. Author manuscript; available in PMC: 2023 Oct 2.
Published in final edited form as: Methods Mol Biol. 2013;1022:129–145. doi: 10.1007/978-1-62703-465-4_11

Fig. 3.

Fig. 3

Chemoenzymatic OGT activity assay. This method involves azide labeling using UDP-GlcNAz, followed by chemoenzymatic reaction either Staudinger ligation with a phosphine reagent as shown in this section, or Click reaction with a terminal alkyne reagent and Cu+1 ion (or strained cycloalkyne without Copper ion catalyst; not provided here)