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. Author manuscript; available in PMC: 2023 Oct 2.
Published in final edited form as: Methods Mol Biol. 2013;1022:129–145. doi: 10.1007/978-1-62703-465-4_11

Fig. 6.

Fig. 6

Ponce S. Stained (a) and Western blot (b) of Nup62. ncOGT utilizes UDP-GlcNAz to label Nup62 giving a distinctive signal after chemoselective reaction with Biotin-Phosphine. Nitrocellulose membrane was probed with IRDye800CW-Streptavidin. Asterisk (*) represents ncOGT in a crude extract of E. coli