Table 3.
Gene name | Forward primer (5′-3′) | Reverse primer (5′-3′) | PCR product (bp) |
---|---|---|---|
β-actin | CTGGCACCTAGCACAATGAA | ACATCTGCTGGAAGGTGGAC | 109 |
ZO-1 | CTTCAGGTGTTTCTCTTCCTCCTC | CTGTGGTTTCATGGCTGGATC | 131 |
ZO-2 | CTCCGTCAGCAGGGAACAA | TTGGGCGTGACGTATAGCTG | 80 |
OCLN | GTCTGTGGGTTCCTCATCGT | GTTCTTCACCCACTCCTCCA | 156 |
CLDN1 | CATACTCCTGGGTCTGGTTGGT | GACAGCCATCCGCATCTTCT | 100 |
CLDN2 | CTGCTCACCCTCATTGGA | AACTCACTCTTGGGCTTCTG | 140 |
CLDN3 | CTTCATCGGCAACAACATCGTGAC | CCAGCATGGAGTCGTACACCTTG | 131 |
SOD1 | ACCAAAAGATGCAGATAGGCAC | GGTACGGCCAATGATGCAGT | 127 |
SOD2 | GGAGGGGAGCCTAAAGGAGAAT | CCCAGCAATGGAATGAGACCT | 215 |
CAT | CGCATGTCCGTTTCAGGAGA | AGATAGAAGTCTCGCACCTGAG | 80 |
GPx | AATTCGGGCACCAGGAGAA | TCACCTCGCACTTCTCGAAC | 114 |
Nrf2 | GATAGTGACCCAGTCTTCATTTC | CTTGGTTTCAGGGCTCGT | 201 |
CAT, catalase; CLDN, Claudin; GPx, glutathione peroxidase; Nrf2, Nuclear factor erythroid 2-related factor 2; OCLN, Occludin; SOD, superoxide dismutase; ZO, Zonula occludens.