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. 2023 Aug 17;18(9):1870–1883. doi: 10.1016/j.stemcr.2023.07.004

Figure 2.

Figure 2

Robust MNPs are derived in 12 days of iPSC differentiation

(A) Representative images of OLIG2+/NKX6.1+ MNPs generated in 12 days of differentiation.

(B) Representative images of OLIG2+/NKX2.2– MNPs generated in 12 days of differentiation.

(C) The proportion of OLIG2+, NKX6.1+, and NKX2.2+ cells.

(D) RT-qPCR data showing relative expression of OLIG2, NKX6.1, PAX6, NGN2, and PAX7 genes in 12-day culture compared to iPSCs. N = 2 cell lines, n = 3 replicates. All representative images were from the control line ALSH84C5, Scale bar, 100 μM. See also Figure S3.