Skip to main content
. 2023 Aug 17;18(9):1870–1883. doi: 10.1016/j.stemcr.2023.07.004

Figure 4.

Figure 4

The iPSC-derived sMNs resemble cervical MMC in the spinal cord

(A) Schematic diagram showing differential expression of OTX2 and HOX genes in the hindbrain and spinal cord. Created with BioRender.com and adapted from Philippidou and Dasen (2013) and Sagner and Briscoe (2019).

(B) Expression of spinal cord positional markers of HOXB4, HOXC5, HOCX6, HOXC8, HOXC9, and HOXC10 in day 0 iPSCs and day 18 iPSC-derived sMNs. N = 3, n = 3.

(C) Schematic diagram presenting MN columnar pattern, cell types, positions, general muscle targets, and specific marker genes. MMC neurons innervate axial muscles, HMC cells innervate hypaxial muscles, PMC neurons innervate diaphragm, LMC neurons innervate limb muscles, and preganglionic motor column (PGC) neurons innervate sympathetic ganglia. Adapted from (Peljto and Wichterle, 2011; Philippidou and Dasen, 2013) and created with BioRender.com.

(D) The expression of FOXP1, LHX3 and POU3F1 mRNA during the course of differentiation. N = 3 cell lines, n = 3 replicates. nd, not detected, as the values were too small to be visible on the graph.