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. 2023 Aug 3;18(9):1827–1840. doi: 10.1016/j.stemcr.2023.07.002

Figure 2.

Figure 2

Flow cytometry analysis of EBs on days 4, 5, and 6

(A and C) Schematic of the gating strategy.

(B) Dot plots displaying expression of VE-CADHERIN and ISL1 in total cells (left panel) and expression of PDGFRa and FLK1 in VE-CADHERIN+/ISL1+ cells (quadrant 2 [Q2] in A, right panel) on days 4, 5, and 6. (B) Density dot plots displaying expression of PDGFRa and FLK1 in total cells (left panel) and expression of VE-CADHERIN and ISL1 in PDGFRa+/FLK1+ cells (Q2 in C, right panel) on days 4, 5, and 6. Numbers in dot plots represent the percentage of frequencies of cell populations in the quadrants (Q1–Q4).

Blue to red color in dot plots (pseudocolor) represents low to high cell frequency, respectively. Representative data from three independent experiments are presented.