Figure 2.

Compromised spatiotemporal assembly of cell-cell junctions in ACM hiPSC-CMs

(A–H) See also Figure S4. Representative immunostained images and averaged heatmaps of ACM hiPSC-CM cell pairs stained for nuclei (blue), α-actinin (red), and cell junction proteins (white). Quantified centers and distributions of intercalated disc protein localization in isogenic WT control and ACM hiPSC-CM cell pairs for cell junction proteins are shown on the right. Mean ± SD. two-way ANOVA followed by Tukey’s multiple comparisons test: ^∗p < 0.05, ^∗∗p < 0.01. Cell pairs were aggregated from three to five independent differentiations.