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. 2023 Aug 17;18(9):1811–1826. doi: 10.1016/j.stemcr.2023.07.005

Figure 4.

Figure 4

SB restores cytoskeletal integrity in day 6 and 9 ACM hiPSC-CMs

(A–F) Day 6 WT and ACM hiPSC-CM cell pairs treated with DMSO (veh) or SB. Representative images of cell pairs stained for F-actin (green) and α-actinin (red), with corresponding skeletonized fibrils pseudo-colored based on angular orientation.

(E) Quantified values for cytoskeletal organization (F-actin and sarcomere OOP) were unaffected, but modest improvement to sarcomere formation (z-disc presence) was observed in ACM + SB (F).

(G–L) Day 9 WT and ACM hiPSC-CM cell pairs treated with veh or SB. Similar trends are observed with more pronounced significant improvements to sarcomere formation in ACM + SB were observed (L). Whereas SB improved cytoskeletal integrity in ACM cell pairs, it reduced these metrics (E, F, K, L) in WT cell pairs. Mean ± SD. Two-way ANOVA followed by Tukey’s multiple comparisons test: ∗p < 0.05. Cell pairs were aggregated from three to five independent differentiations.