Figure 6.

Effect of bumetanide on post-injury microglia surveillance monitored by two-photon in vivo imaging. (A) Schematic representation of the experimental timeline. (B) Maximal projection of image at 1 and 5 days after laser-induced focal injury. Bottom: A single microglia cell and the corresponding temporal colour-coded projection (recording time of 1 h at 5 min intervals). (C) Quantification of process length at 1 and 5 days after laser-induced focal injury in the presence and absence of bumetanide. (D) As for B in bumetanide (Bum)-treated animals. Bottom: A single microglia and the corresponding temporal colour-coded projection (recording time of 1 h at 5 min intervals). (E) Quantification of motility ratio at 1 and 5 days in the presence and absences of bumetanide. All data were analysed using unpaired Student's t-test. *P < 0.05.