Fig. 7. Highly correlated signaling in axons and somas within genetic subtypes of dopamine neurons.
a, Mouse running on treadmill during dual fiber photometry. b, Schematic of simultaneous photometry recordings from SNc and striatum. c, Example recordings for DAT and each subtype showing simultaneous fluorescence traces (ΔF/F) from SNc and striatum. Isosbestic controls in blue. ▼, example transients present in SNc and in striatum; ▽, example transient present in striatum but not in SNc (white fill) or vice versa (gray fill). d, Cross-correlation between ΔF/F traces from striatum and SNc shown in c. Isosbestic controls in blue. e, Average cross-correlation between simultaneous ΔF/F traces from striatum and SNc for all recordings of each subtype and DAT. Isosbestic controls in blue. Shaded regions denote mean ± s.e.m. across recordings. Heat map shows cross-correlations for each paired recording sorted by peak magnitude. DAT mice = 5, n = 35 recordings; Vglut2 mice = 4, n = 11 recordings; Calb1 mice = 2, n = 5 recordings; Anxa1 mice = 8, n = 43 recordings. f, Distribution of peak cross-correlations between SNc and striatum for recordings of all subtypes and DAT shown in e. P values for comparison to DAT: Vglut2 = 3 × 10−4, Calb1 = 3 × 10−3, Anxa1 = 3 × 10−4 (two-sided Mann–Whitney U-test with Bonferroni correction). Ave, average; Cross-corr, cross-correlation; Str, striatum.