Skip to main content
. 2023 May 5;146(10):4217–4232. doi: 10.1093/brain/awad148

Figure 2.

Figure 2

Alternative splicing regulation during ChP development. (A) Developmental stage- (E13.5, P1, P14, adult) specific variations in size and morphology of LVChP. Scale = 1 mm. (B) Plot of AS events (Ψ) in wild-type FVB mice ChP comparing E13.5 to adult per cent spliced in (PSI) with the significant events in blue. (C) Numa1 exon 15 AS RNA-seq reads during development (E13.5, P0, P14, adult, light grey to black gradient). (D) RT-PCR gels (representative) and analysis (bar graph) validations for E13.5, P0, P14 and adult stages of Numa1 exon 15, Picalm exon 18, Postn exon 18 and Ndrg2 exon 3. (E) In contrast to other splicing factors, Mbnl2 expression increases during postnatal development. Relative expression (RNA-seq) is plotted for significant differentially expressed genes during LVChP development. (F) The ratio of Dmpk/Mbnl expression was calculated as the normalized expression of Dmpk/(Mbnl1 + Mbnl2 + Mbnl3) for each developmental stage. #Significance of at least P < 0.05 for all the compared stages, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, one-way ANOVA. LVChP = lateral ventricle choroid plexus.