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. 2023 May 24;44(10):2004–2018. doi: 10.1038/s41401-023-01101-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Representative image of the comet assay and immunohistochemistry of γ-H2AX and 53BP1 in the Fgf10^+/− and Fgf10^+/+ mice (×400 magnification). b Statistical analysis of the comet assay, γ-H2AX and 53BP1 presented in a. The comet assay was used to assess DNA damage through %DNA in tail and tail moment. The results of immunohistochemistry are shown as positive nucleus in all nuclei (per nucleus). c Representative image of the comet assay and immunohistochemistry of γ-H2AX and 53BP1 in WT mice from indicated groups (×400 magnification). d Statistical analysis of the comet assay, γ-H2AX and 53BP1 presented in c. e Representative image of the comet assay in HL-1 cells. f Statistical analysis of the comet assay in HL-1 cells. g Images of γ-H2AX and 53BP1 foci at different time points. h Statistical analysis of the number of γ-H2AX and 53BP1 foci. ns non-significant; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. γH2AX gamma-H2A histone family member X, 53BP1 p53-binding protein 1, WT wild type. Scaler bar: 100 μm.