Skip to main content
. 2023 May 24;44(10):2004–2018. doi: 10.1038/s41401-023-01101-x

Fig. 7. FGF10 exerts cardioprotective effects via the PHLDA1/Akt axis.

Fig. 7

a Western blot analysis of phospho-Akt and total Akt, as well as PHLDA1 in the indicated groups of mice. b Western blot analysis of phospho-, total Akt and PHLDA1 in HL-1 cells. c, d Statistical analysis of the immunoblot data presented in a and b, respectively. e PHLDA1 mRNA expression in mice with RT-qPCR. f Western blot analysis of total and phospho-AKT, SOD, and C-CAS3 in HL-1 cells with or without MK2206. g Immunoprecipitation against PHLDA1 was performed with pre-conjugated anti-HA agarose beads using 293T protein extracts to determine its interaction with phospho-FGFR2. h Western blot analysis of total and phospho-AKT, SOD, and C-CAS3 in HL-1 cells transfected with SH-PHLDA1 or OE-PHLDA1. i Cartoon diagram summarizing our findings on FGF10-mediated protection against doxorubicin-induced toxicity. PHLDA1 Pleckstrin homology-like domain family A member 1, ns non-significant; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.