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. 2023 Oct 2;13:16560. doi: 10.1038/s41598-023-43678-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Passing-Bablok regression graph showing the agreement between concentration measured with a haemocytometer (Hem) versus total events estimated with volumetric flow cytometry (volFC-CevT), events positive to Hoechst 33,342 (volFC-CevH +), events gated on sperm morphological criteria (volFC-CevM), and events gated on both sperm morphology and Hoechst positivity (volFC-CevM/H +). The dotted brown line represents the 45°-identity line (x = y), the solid blue line represents the regression line, and the dashed brown lines represent the confidence interval for the regression line. Note that Hem vs volFC-CevM/H + overlap closely with the 45 °-identity line, and Hem vs volFC-CevT is more divergent.