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. 2023 Jun 21;44(10):2075–2090. doi: 10.1038/s41401-023-01113-7

Fig. 2. PXR activation by PCN attenuates renal fibrosis induced by adenine.

Fig. 2

a Masson trichrome staining and immunostaining showing that PCN treatment significantly attenuated renal extracellular matrix protein deposition and the protein expression of fibronectin, αSMA and collagen I. Scale bar: 100 μm. b–e Semiquantitative analysis of a (n = 4–6). f Western blot analysis demonstrating that PCN treatment markedly reduced adenine-induced expression of fibrosis-related proteins including p-Smad3, fibronectin and αSMA (n = 3). g Semiquantitative measurement for the protein levels in f. h Immunofluorescence assay showing that PCN treatment significantly inhibited adenine-induced activation of p-Smad3. Scale bar: 50 μm. i Semiquantitative measurement for the protein levels in h (n = 3). Data represents the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001.