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. 2023 Jun 21;44(10):2075–2090. doi: 10.1038/s41401-023-01113-7

Fig. 7. PXR activation inhibits the Wnt7a/β-catenin signaling in the kidneys of adenine-treated mice.

Fig. 7

a Real-time PCR analysis demonstrating the effect of PCN treatment on the expression of all 19 Wnt members in mice treated with adenine for 4 weeks. Adenine induced ~13-fold increase in Wnt7a expression, which was significantly attenuated by PCN treatment (n = 4–8). b, c Western blot assay and semiquantitative analysis indicate that PCN treatment significantly decreased adenine-induced protein levels of Wnt7a and its downstream effector β-catenin (n = 3). d Immunofluorescence assay showed that PCN treatment inhibited adenine-induced activation of β-catenin. Scale bar: 50 μm. Results are expressed as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001.