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. 2023 Jun 21;44(10):2075–2090. doi: 10.1038/s41401-023-01113-7

Fig. 8. PXR activation and overexpression inhibit TGFβ1-induced fibrotic gene expression in cultured MTEC.

Fig. 8

a Western blot analysis demonstrating that PXR activation decreased TGFβ1-induced upregulation of p-Smad3, fibronectin and αSMA protein expression in MTEC. b Semiquantitative analysis of the protein levels in a (n = 4). c Immunofluorescence assay showed that PXR activation attenuated TGFβ1-induced activation of p-Smad3. Scale bar: 25 μm. d Western blot analysis demonstrated that adenovirus-mediated PXR overexpression decreased TGFβ1-induced p-Smad3, fibronectin and αSMA expression in MTEC. e Semiquantitative analysis of the protein levels in d (n = 4). f Immunofluorescence assay showed that PXR overexpression abolished the activation of p-Smad3 induced by TGFβ1. Scale bar: 25 μm. Results are expressed as mean ± SEM. **P < 0.01, ***P < 0.001.