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. 2023 May 23;44(10):2019–2036. doi: 10.1038/s41401-023-01105-7

Fig. 1. Theaflavin inhibits ATP- or nigericin-induced NLRP3 inflammasome activation in macrophages.

Fig. 1

J774A.1 cells or bone-marrow-derived macrophages (BMDMs) were first primed with LPS (500 ng/mL) for 4 h and then pretreated with indicated doses of theaflavin for 1 h, followed by incubation with ATP (4 mM) for 1 h (J774A.1) (b), ATP (4 mM) for 30 min (BMDMs) (c) or nigericin (5 µM) for 1 h (BMDMs) (d) in the absence of LPS. a The chemical structure of theaflavin. Western blot analysis of caspase-1 and IL-1β in the culture supernatants and indicated proteins in cell lysates from J774A.1 cells treated with ATP (b) or BMDMs treated with ATP (c) or nigericin (d). β-Actin was used as a loading control for cell lysates. TF theaflavin, MCC MCC950, CASP1 caspase-1.