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. 2023 May 23;44(10):2019–2036. doi: 10.1038/s41401-023-01105-7

Fig. 8. MSU-induced peritonitis is alleviated by theaflavin treatment.

Fig. 8

C57BL/6 J mice were administered intragastrically (i.g.) with theaflavin (250 mg/kg body weight) or vehicle (2% Tween 80 in PBS) once a day for three consecutive days before intraperitoneal injection with sterile PBS (0.2 mL) or with 1 mg MSU (in 0.2 mL sterile PBS). One hour later, mice were administered with vehicle or theaflavin (i.g.) once again. Neutrophil and soluble cytokine levels in the peritoneal lavage fluids were quantified 6 h after MSU administration (n = 6 mice per group). a Representative flow cytometric dot-plots of peritoneal exudate cells. b, c Quantification of neutrophils in the peritoneal lavage fluids. d, e IL-1β and IL-6 levels in peritoneal lavage fluid supernatants were measured by cytometric bead array (CBA) assay. Data are shown as mean ± SD (n = 6). ***P < 0.001; TF theaflavin.