Testing for anti-sperm antibodies in serum of either males or females with unexplained infertility is not recommended (Menge et al., 1982; Mardesic et al., 2000; Monem and Moalla, 2003; Yasin et al., 2016).	Strong ⊕○○○
Testing for coeliac disease in women with unexplained infertility can be considered (Tersigni et al., 2014).	Conditional ⊕⊕○○
Testing for thyroid antibody and other autoimmune conditions (apart from coeliac disease) in women with unexplained infertility is not recommended (Poppe et al., 2002; Abalovich et al., 2007; Kilic et al., 2008).	Strong ⊕○○○
TSH measurement is considered good practice in pre-conception care.	GPP
No additional thyroid evaluation in women is recommended if TSH is within the normal range (Duran et al., 2013; Unuane et al., 2013; Orouji Jokar et al., 2018; Rehman et al., 2020).	Strong ⊕○○○
Testing for thrombophilia in women with unexplained infertility is not recommended (Behjati et al., 2006; Bellver et al., 2008; Coulam and Jeyendran, 2009; Casadei et al., 2010; Fatini et al., 2012; Steinvil et al., 2012; Kydonopoulou et al., 2017; Milenkovic et al., 2020).	Strong ⊕○○○
Measurement of oxidative stress in semen of males with unexplained infertility should only be considered in the context of research.	Research only
Measurement of oxidative stress in women with unexplained infertility is not recommended (Veena et al., 2008; Pekel et al., 2015; Lazzarino et al., 2021; Şentürk et al., 2021).	Strong ⊕⊕○○
Genetic or genomic tests are currently not recommended in couples with unexplained infertility (Trková et al., 2000; Papanikolaou et al., 2005; Witkin et al., 2010; Sahmani et al., 2011; Vani et al., 2012; Suganya et al., 2015; Salas-Huetos et al., 2016; Rull et al., 2018; Ertosun et al., 2022).	Strong ⊕○○○
Testing for vitamin D deficiency in women is not recommended for diagnosis of unexplained infertility (Rudick et al., 2012; Lopes et al., 2017; Butts et al., 2019; Güngör et al., 2022; Ko et al., 2022).	Strong ⊕○○○
Prolactin testing in women is not recommended (Subramanian et al., 1997; Veena et al., 2008; Orouji Jokar et al., 2018; Qu et al., 2020).	Strong ⊕○○○
BMI measurement in women is considered good practice in pre-conception care.	GPP