TABLE 3.

Outcross and incross of flqA (grlB543) locus by transformation with chromosomal DNA

Donor		Recipient		MIC (μg/ml) for recipient:		Phenotype of transformanta		No. of trans- formants
Strain	Genotype	Strain	Genotype	Ciprofloxacin	Novobiocin	Ciprofloxacin	Novobiocin
ISP2133	trp-489 Ω(chr::Tn917lac)2	MT5224c9	nov (gyrB142) grlB543	1.0b	5.0c	R	S	137
						S	R	109
EN3	grlB543 Ω(chr::Tn917lac)2	MT5	nov (gyrB142)	0.25c	40.0b	S	R	92
						R	S	57

^aS, susceptible; R, resistant. The phenotype was determined by plating each colony on ciprofloxacin at 0.125, 0.5, and 1.0 μg/ml and on novobiocin at 1.25 and 20 μg/ml. For scoring transformants for ciprofloxacin susceptibility, R was defined as growth at 0.125 and 0.5 μg/ml and no growth at 1.0 μg/ml and S was defined as growth at 0.125 μg/ml and no growth at 0.5 and 1.0 μg/ml. For scoring transformants for novobiocin susceptibility, R was defined as growth at 1.25 and 20 μg/ml and S was defined as growth at 1.25 μg/ml and no growth at 20 μg/ml. 

^bValue indicates resistance. 

^cValue indicates susceptibility.